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The method comprises introducing into the prokaryotic organism a nucleic acid comprising a nucleotide sequence encoding an essential gene under the control of at least one tet operator, nizoral shampoo uk iteratively
and an expressible nucleic acid encoding a modified tetracycline repressor, wherein said modified tetracycline repressor binds to a tetracycline operator sequence in the prokaryotic organism with a greater affinity in the presence of tetracycline or a tetracycline analog than in the absence of tetracycline or a tetracycline analog; culturing the prokaryotic organism under conditions such that the modified tetracycline repressor is expressed and in the presence of tetracycline or tetracycline analog at a concentration sufficient to repress expression of the essential gene; contacting the prokaryotic organism with a test compound; and determining the effect of the test compound compared to control cell not cultured in tetracycline or tetracycline analog.